What this means
Scientists built stem cell lines that can be turned on to make progerin, a protein linked to fast aging. This shows the cells can be used as a model, but it does not show any aging effects yet.
Plain-English summary written by an AI model from the abstract. The original is below.
- Who it was tested in
- Human stem cells grown in a lab dish
- Kind of research
- A study
The science
- Original title
- Generation of inducible LMNA and Progerin hiPSC lines for controlled modelling of cellular ageing.
- Authors
- Veleva D, Moreno CL, Clarke M et al.
- Journal
- Stem cell research
- Published
- Oct 13, 2026
- Study type
- Study
- PMID
- 42868746 ↗
Abstract
Modelling human ageing in vitro remains challenging due to the limited ability of hiPSC systems to recapitulate progressive cellular decline. We generated hiPSC lines with a dual-cassette doxycycline-inducible system enabling controlled Progerin expression as a model of accelerated ageing, alongside matched inducible LMNA and GFP control lines. TALEN-mediated genome editing generated a Tet-On system comprising a constitutive M2rtTA regulatory cassette and TRE-driven transgene cassette targeted to the AAVS1 locus. Targeted integration was confirmed by PCR and sequencing confirmed the Progerin-specific deletion. The engineered lines retained pluripotency and demonstrated strong inducible expression, providing a versatile platform for modelling ageing-associated cellular phenotypes.
Veleva D, Moreno CL, Clarke M et al. Generation of inducible LMNA and Progerin hiPSC lines for controlled modelling of cellular ageing. Stem cell research. 2026. doi:10.1016/j.scr.2026.104112. PMID: 42868746. https://pubmed.ncbi.nlm.nih.gov/42868746/