What this means
This trial plans to test whether mild water loss changes inflammation, blood sugar, sleep, and strength in middle-aged adults. It has not reported results, so it cannot yet show any effect.
Plain-English summary written by an AI model from the registry listing.
- Status
- Recruiting
- Who can join
- Ages 45–65
- Where
- 1 site, including Texas
The science
- Registered title
- Dehydration, Rehydration, and Impact on Physiology
- Trial ID
- NCT07084675 ↗
- Phase
- Not applicable
- Study type
- interventional
- Conditions
- Aging
- What is tested
- Hydration-Dehydration-Rehydration Protocol (HDR)
- Sponsor
- Texas Tech University
- Ages
- 45 Years to 65 Years
- Dates
- First posted Jul 24, 2025 · updated Sep 17, 2025
Registry summary
Our goal is to determine whether hydration status influences inflammation, and overall physiological performance in adults aged 45-65.
Aim 1: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on H2O2, upstream inflammatory signal (NFkB), mitochondrial fragmentation, and autophagy in circulating PBMCs.
Aim 2: The effects of 4-day mild dehydration followed by 4-day rehydration (defined as approximately 2-3% body mass loss) on fasting glucose, sleep performance, and strength performance.
Participant will:
complete a 4-day hydration protocol, followed by a 4-day dehydration protocol, and then a 4-day rehydration protocol.
attend five laboratory visits for testing: Day 1: Informed consent and familiarization Day 2: Pre-hydration testing (before the 4-day hydration protocol) Day 3: Pre-dehydration testing (before the dehyration protocol) Day 4: Post-dehydration testing (after the dehydration protocol) Day 5: Post-rehydration testing (after the rehydration protocol)
Sites (1)
- Texas Tech University, Lubbock, Texas, United States